Експресія білка refers to the synthesis of білки within cells using information contained in the DNA or gene.
Білки are responsible for all the biochemical reactions that take place within the cell. Hence, it makes it necessary to study білок function in order to understand cellular processes.
This is currently studied based on using fluorescent білки as tags. However, this does not provide real time analysis as it requires maturation of chromophore that takes time and this causes a delay in investigation of real-time expression, especially for білки які носять тимчасовий або ефемерний характер.
Дослідники повідомили про нову техніку на сервері препринтів 30 липня 2020 року, яка може подолати це обмеження.
The new study describes the use a fluorescent biosensor that enables real-time detection of білок expression in vivo which has implications for understanding the spatiotemporal expression of білки within the living organism. This sensor is based on a dim green fluorescent білок in which pre-existing fluorescence increases 11-fold in vivo following the specific and rapid binding of a білок tag and enables detection of білок expression within seconds in live cells.
це біосенсор will be useful to study biological processes in real-time where білки are either transiently expressed and/or to understand the expression and interactions of білки from a disease-causing organism such as bacteria or a virus with that of the host proteins.
Посилання:
Ісон М.Г., Панделєва А.Т., Майєр М.М. та ін. 2020. Генетично закодований флуоресцентний біосенсор для швидкого виявлення експресії білка. Препринт: bioRxiv 2020.07.30.229633; DOI: https://doi.org/10.1101/2020.07.30.229633
***
